Overview
Our 96-Well Ras Activation ELISA Kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.
Product Line
Cell Signaling and Protein Biology.
Host
N/A.
Species Reactivity
N/A.
Shipping Temperature
2 parts: 1 RT / 1 Blue Ice.
Product Classification
Cell Signaling and Protein Biology; G Protein Signaling / Small GTPase Assays and Reagents; Small GTPase Research Tools; Small GTPase Activation ELISA Kits; Ras Activation ELISA, Colorimetric.
Detection Method
Colorimetric.
Application
N/A.
Storage Temperature
Upon receipt, aliquot and store Raf-1 RBD at -80ºC and avoid freeze/thaw. Aliquot and store the anti-pan-Ras Antibody, Secondary Antibody, GTPγS, and GDP components at -20ºC and avoid freeze/thaw. Store all other components at 4ºC until their expiration dates.