AffiELISA® Pan HVD3 Kit: Max Sensitivity 25-Hydroxyvitamin D3 ELISA Detection

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CAT# AFG-E0031
Size: 96 Tests

348.00 348.0 USD 348.00

348.00

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The AffiELISA® Pan HVD3 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with 25-Hydroxyvitamin D3 (HVD3) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3 (HVD3) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of 25-Hydroxyvitamin D3 (HVD3) in the samples can be determined.

AffiELISA® Pan HVD3 Kit

25-Hydroxyvitamin D3

25-HVD3 - 25HVD3 - Calcifediol - Calcidiol - H-VD3 - 25-Hydroxycholecalciferol - 25-Hydroxy Vitamin D3 - 25 (OH) D

Pan

Competitive Inhibition ELISA

Serum, plasma and other biological fluids

Reproductive science, Genetic science, Nutrition metabolism, Bone metabolism.