AffiELISA® Pan ABA Kit: Max Sensitivity Abscisic Acid ELISA Detection

The AffiELISA® Pan ABA Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Abscisic Acid (ABA) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Abscisic Acid (ABA) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Abscisic Acid (ABA) in the samples can be determined.