AffiELISA® Mouse ADCYAP1 Kit: Ultra-Sensitive Adenylate Cyclase Activating Polypeptide 1, Pituitary ELISA Detection

The AffiELISA® Mouse ADCYAP1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples can be determined.