AffiELISA® Human ADCYAP1 Kit: Super-Sensitive Adenylate Cyclase Activating Polypeptide 1, Pituitary ELISA Detection

The AffiELISA® Human ADCYAP1 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples can be determined.