AffiELISA® Human AOPP Kit: High-Resolution Advanced Oxidation Protein Products ELISA Detection

The AffiELISA® Human AOPP Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Advanced Oxidation Protein Products (AOPP) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Advanced Oxidation Protein Products (AOPP) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Advanced Oxidation Protein Products (AOPP) in the samples can be determined.