AffiELISA® Rat ACCPA Kit: High Sensitivity anti-cyclic citrullinated peptide antibody ELISA Detection

The AffiELISA® Rat ACCPA Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to anti-cyclic citrullinated peptide antibody (ACCPA) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to anti-cyclic citrullinated peptide antibody (ACCPA) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain anti-cyclic citrullinated peptide antibody (ACCPA) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of anti-cyclic citrullinated peptide antibody (ACCPA) in the samples can be determined.