The AffiELISA® Human TPO-Ab Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to anti-Thyroid-Peroxidase antibody (TPO-Ab) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to anti-Thyroid-Peroxidase antibody (TPO-Ab) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain anti-Thyroid-Peroxidase antibody (TPO-Ab) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of anti-Thyroid-Peroxidase antibody (TPO-Ab) in the samples can be determined.
AffiELISA® Human TPO-Ab Kit
anti-Thyroid-Peroxidase antibody
TPO Ab - TPO-Ab
Human
Sandwich ELISA
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Signal transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology.