AffiELISA® Human AP12 Kit: High Sensitivity Apelin 12 ELISA Detection

The AffiELISA® Human AP12 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Apelin 12 (AP12) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Apelin 12 (AP12) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Apelin 12 (AP12) in the samples can be determined.