The AffiELISA® Human ADMA Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Asymmetrical Dimethylarginine (ADMA) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Asymmetrical Dimethylarginine (ADMA) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Asymmetrical Dimethylarginine (ADMA) in the samples can be determined.
AffiELISA® Human ADMA Kit
Asymmetrical Dimethylarginine
N,N-Dimethylarginine - NG,NG-Dimethylarginine dihydrochloride - Asymmetric Dimethylarginine
Human
Competitive Inhibition ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Metabolic pathway, Cardiovascular biology.