AffiELISA® Human BPI Kit: High Sensitivity Bactericidal/Permeability Increasing Protein ELISA Detection

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CAT# AFG-E0926
Size: 96 Tests

389.00 389.0 USD 389.00

389.00

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The AffiELISA® Human BPI Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Bactericidal/Permeability Increasing Protein (BPI) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Bactericidal/Permeability Increasing Protein (BPI) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Bactericidal/Permeability Increasing Protein (BPI) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Bactericidal/Permeability Increasing Protein (BPI) in the samples can be determined.

AffiELISA® Human BPI Kit

Bactericidal/Permeability Increasing Protein

Human

Sandwich ELISA

Serum, plasma, tissue homogenates and other biological fluids

Infection immunity, Dermatology.