The AffiELISA® Human CACNa1D Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) in the samples can be determined.
AffiELISA® Human CACNa1D Kit
Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit
CACH3 - CACN4 - CACNL1A2 - CCHL1A2 - Cav1.3 - Voltage-gated calcium channel subunit alpha Cav1.3 - Calcium channel, L type, alpha-1 polypeptide, isoform 2
Human
Sandwich ELISA
Tissue homogenates and other biological fluids.
Signal transduction.