The AffiELISA® Human CASR Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Calcium Sensing Receptor (CASR) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Calcium Sensing Receptor (CASR) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Calcium Sensing Receptor (CASR) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Calcium Sensing Receptor (CASR) in the samples can be determined.
AffiELISA® Human CASR Kit
Calcium Sensing Receptor
CAR - FHH - FIH - GPRC2A - HHC - HHC1 - NSHPT - PCAR1 - Parathyroid cell calcium-sensing receptor 1 - Hypocalciuric Hypercalcemia 1 - Severe Neonatal Hyperparathyroidism
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Metabolic pathway, Endocrinology, Neuroscience, Hormone metabolism.