AffiELISA® Human CAMK2b Kit: Super-Sensitive Calcium/Calmodulin Dependent Protein Kinase II Beta ELISA Detection

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CAT# AFG-E1217
Size: 96 Tests

389.00 389.0 USD 389.00

389.00

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The AffiELISA® Human CAMK2b Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in the samples can be determined.

AffiELISA® Human CAMK2b Kit

Calcium/Calmodulin Dependent Protein Kinase II Beta

CAM2 - CAMK2 - CAMKB - Proline Rich Calmodulin-Dependent Protein Kinase

Human

Sandwich ELISA

Tissue homogenates, cell lysates and other biological fluids.

Enzyme and Kinase.