The AffiELISA® Human CAMKK2 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) in the samples can be determined.
AffiELISA® Human CAMKK2 Kit
Calcium/Calmodulin Dependent Protein Kinase Kinase 2
CAMKK - CAMKKB - Calcium/calmodulin-dependent protein kinase kinase beta
Human
Sandwich ELISA
Tissue homogenates and other biological fluids.
Enzyme and Kinase.