The AffiELISA® Human CA125 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Carbohydrate Antigen 125 (CA125) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Carbohydrate Antigen 125 (CA125) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 125 (CA125) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Carbohydrate Antigen 125 (CA125) in the samples can be determined.
AffiELISA® Human CA125 Kit
Carbohydrate Antigen 125
MUC16 - Cancer Antigen 125 - Mucin 16,Cell Surface Associated - Ovarian Cancer-Related Tumor Marker CA125 - Ovarian Carcinoma Antigen CA125
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Tumor immunity, Reproductive science.