The AffiELISA® Human CA50 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Carbohydrate Antigen 50 (CA50) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Carbohydrate Antigen 50 (CA50) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 50 (CA50) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Carbohydrate Antigen 50 (CA50) in the samples can be determined.
AffiELISA® Human CA50 Kit
Carbohydrate Antigen 50
cancer Antigen 50
Human
Sandwich ELISA
Serum, plasma and other biological fluids
Tumor immunity, Gastroenterology.