The AffiELISA® Human CA9 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Carbonic Anhydrase IX (CA9) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Carbonic Anhydrase IX (CA9) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase IX (CA9) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Carbonic Anhydrase IX (CA9) in the samples can be determined.
AffiELISA® Human CA9 Kit
Carbonic Anhydrase IX
CA-IX - CAIX - MN - pMW1 - P54/58N - Carbonic Dehydratase - Renal cell carcinoma-associated antigen G250 - RCC-Associated Protein G250 - Carbonate dehydratase IX
Human
Sandwich ELISA
Serum, plasma, tissue homogenates and other biological fluids.
Enzyme and Kinase.