AffiELISA® Human CA6 Kit: Enhanced Carbonic Anhydrase VI ELISA Detection

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CAT# AFG-E1297
Size: 96 Tests

389.00 389.0 USD 389.00

389.00

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The AffiELISA® Human CA6 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Carbonic Anhydrase VI (CA6) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Carbonic Anhydrase VI (CA6) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VI (CA6) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Carbonic Anhydrase VI (CA6) in the samples can be determined.

AffiELISA® Human CA6 Kit

Carbonic Anhydrase VI

CA-VI - CAVI - GUSTIN - Carbonate dehydratase VI - Salivary carbonic anhydrase - Secreted carbonic anhydrase

Human

Sandwich ELISA

Serum, plasma, saliva and other biological fluids

Enzyme and Kinase.