AffiELISA® Pan CML Kit: Max Sensitivity Carboxymethyl Lysine ELISA Detection

The AffiELISA® Pan CML Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Carboxymethyl Lysine (CML) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Carboxymethyl Lysine (CML) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Carboxymethyl Lysine (CML) in the samples can be determined.