The AffiELISA® Human CASP1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Caspase 1 (CASP1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Caspase 1 (CASP1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Caspase 1 (CASP1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Caspase 1 (CASP1) in the samples can be determined.
AffiELISA® Human CASP1 Kit
Caspase 1
ICE - IL1BC - IL1-BC - P45 - Interleukin 1 Beta Converting Enzyme - Apoptosis-Related Cysteine Peptidase - Interleukin-1 beta convertase
Human
Sandwich ELISA
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Signal transduction, Enzyme and Kinase, Apoptosis, Infection immunity.