The AffiELISA® Human CASP9 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Caspase 9 (CASP9) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Caspase 9 (CASP9) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Caspase 9 (CASP9) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Caspase 9 (CASP9) in the samples can be determined.
AffiELISA® Human CASP9 Kit
Caspase 9
APAF3 - ICE-LAP6 - MCH6 - Apoptosis-Related Cysteine Peptidase - Cysteinyl Aspartate Specific Proteinases 9 - Apoptotic protease-activating factor 3 - ICE-like apoptotic protease 6
Human
Sandwich ELISA
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Signal transduction, Enzyme and Kinase, Apoptosis, Tumor immunity, Infection immunity.