The AffiELISA® Human CAV2 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Caveolin 2 (CAV2) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Caveolin 2 (CAV2) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Caveolin 2 (CAV2) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Caveolin 2 (CAV2) in the samples can be determined.
AffiELISA® Human CAV2 Kit
Caveolin 2
CAV - CAV2 - CAV2_HUMAN - Caveolae protein 20 kD - Caveolin 2 - Caveolin 2 isoform a and b - Caveolin-2 -
Human
Sandwich ELISA
Serum, plasma, tissue homogenates and other biological fluids
Tumor immunity.