AffiELISA® Human CGa Kit: High-Resolution Chorionic Gonadotropin Alpha Polypeptide ELISA Detection

The AffiELISA® Human CGa Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Chorionic Gonadotropin Alpha Polypeptide (CGa) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the samples can be determined.