The AffiELISA® Human CBX3 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Chromobox Homolog 3 (CBX3) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Chromobox Homolog 3 (CBX3) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Chromobox Homolog 3 (CBX3) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Chromobox Homolog 3 (CBX3) in the samples can be determined.
AffiELISA® Human CBX3 Kit
Chromobox Homolog 3
HECH - HP1-GAMMA - HP1Hs-Gamma - HP1 Gamma Homolog,Drosophila - Heterochromatin protein 1 homolog gamma - Modifier 2 protein
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids.
Signal transduction, Developmental science.