The AffiELISA® Human C16orf89 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Chromosome 16 Open Reading Frame 89 (C16orf89) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Chromosome 16 Open Reading Frame 89 (C16orf89) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Chromosome 16 Open Reading Frame 89 (C16orf89) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Chromosome 16 Open Reading Frame 89 (C16orf89) in the samples can be determined.
AffiELISA® Human C16orf89 Kit
Chromosome 16 Open Reading Frame 89
C16orf89
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids.
Signal transduction.