The AffiELISA® Human CLL1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Collectin Liver 1 (CLL1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Collectin Liver 1 (CLL1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Collectin Liver 1 (CLL1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Collectin Liver 1 (CLL1) in the samples can be determined.
AffiELISA® Human CLL1 Kit
Collectin Liver 1
COLEC10 - CL-L1 - CL-34 - Collectin Sub-Family Member 10 - C-Type Lectin - Collectin-10 - Collectin-34
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Signal transduction, CD and Adhesion molecule, Developmental science.