The AffiELISA® Human CCA Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Colon Cancer Antigen (CCA) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Colon Cancer Antigen (CCA) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Colon Cancer Antigen (CCA) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Colon Cancer Antigen (CCA) in the samples can be determined.
AffiELISA® Human CCA Kit
Colon Cancer Antigen
Colon Cancer Antigen
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Cancer.