The AffiELISA® Human C4B Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Complement C4-B (C4B) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Complement C4-B (C4B) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Complement C4-B (C4B) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Complement C4-B (C4B) in the samples can be determined.
AffiELISA® Human C4B Kit
Complement C4-B
CO4 - CPAMD3 - Basic complement C4 - C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Human
Sandwich ELISA
Serum, plasma and other biological fluids
Metabolic pathway, Infection immunity, Immune molecule.