AffiELISA® Human C3a Kit: High Sensitivity Complement Component 3a ELISA Detection

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CAT# AFG-E1934
Size: 96 Tests

389.00 389.0 USD 389.00

389.00

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The AffiELISA® Human C3a Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Complement Component 3a (C3a) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Complement Component 3a (C3a) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Complement Component 3a (C3a) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Complement Component 3a (C3a) in the samples can be determined.

AffiELISA® Human C3a Kit

Complement Component 3a

Complement Component 3a

Human

Sandwich ELISA

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Infection immunity, Immune molecule, Autoimmunity.