The AffiELISA® Human C4a Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Complement Component 4a (C4a) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Complement Component 4a (C4a) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Complement Component 4a (C4a) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Complement Component 4a (C4a) in the samples can be determined.
AffiELISA® Human C4a Kit
Complement Component 4a
C4a anaphylatoxin
Human
Sandwich ELISA
Serum, plasma and other biological fluids
Immune molecule, Endocrinology.