The AffiELISA® Human C5aR1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Complement Component 5a Receptor 1 (C5aR1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Complement Component 5a Receptor 1 (C5aR1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Complement Component 5a Receptor 1 (C5aR1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Complement Component 5a Receptor 1 (C5aR1) in the samples can be determined.
AffiELISA® Human C5aR1 Kit
Complement Component 5a Receptor 1
CD88 - C5A - C5-AR1 - C5R1 - C5a anaphylatoxin chemotactic receptor 1
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Signal transduction, Infection immunity, Immune molecule.