AffiELISA® Human CAR Kit: High-Resolution Constitutive Androstane Receptor ELISA Detection

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CAT# AFG-E2000
Size: 96 Tests

389.00 389.0 USD 389.00

389.00

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The AffiELISA® Human CAR Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Constitutive Androstane Receptor (CAR) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Constitutive Androstane Receptor (CAR) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Constitutive Androstane Receptor (CAR) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Constitutive Androstane Receptor (CAR) in the samples can be determined.

AffiELISA® Human CAR Kit

Constitutive Androstane Receptor

NR1I3 - NR1-I3 - CAR1 - MB67 - Nuclear Receptor Subfamily 1,Group I,Member 3 - Constitutive active response - Constitutive activator of retinoid response - Orphan nuclear receptor MB67

Human

Sandwich ELISA

Tissue homogenates, cell lysates and other biological fluids

Signal transduction, Metabolic pathway, Endocrinology.