The AffiELISA® Human CDK5 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Cyclin Dependent Kinase 5 (CDK5) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Cyclin Dependent Kinase 5 (CDK5) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 5 (CDK5) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Cyclin Dependent Kinase 5 (CDK5) in the samples can be determined.
AffiELISA® Human CDK5 Kit
Cyclin Dependent Kinase 5
PSSALRE - CDKN5 - Cell division protein kinase 5 - Serine/threonine-protein kinase PSSALRE - Tau protein kinase II catalytic subunit - TPKII catalytic subunit
Human
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids.
Signal transduction, Enzyme and Kinase, Neuroscience, Developmental science.