AffiELISA® Human CYP3A4 Kit: Enhanced Cytochrome P450 3A4 ELISA Detection

The AffiELISA® Human CYP3A4 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Cytochrome P450 3A4 (CYP3A4) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Cytochrome P450 3A4 (CYP3A4) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A4 (CYP3A4) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Cytochrome P450 3A4 (CYP3A4) in the samples can be determined.