The AffiELISA® Human CAP3 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Cytoplasmic Antiproteinase 3 (CAP3) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Cytoplasmic Antiproteinase 3 (CAP3) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Cytoplasmic Antiproteinase 3 (CAP3) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Cytoplasmic Antiproteinase 3 (CAP3) in the samples can be determined.
AffiELISA® Human CAP3 Kit
Cytoplasmic Antiproteinase 3
SERPINB9 - PI9 - Serpin Peptidase Inhibitor Clade B Member 9 - Peptidase inhibitor 9
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Infection immunity.