AffiELISA® Pan 3,4-dihydroxyphenyl acetic acid  Kit: Ultra-High DOPAC ELISA Detection

The AffiELISA® Pan 3,4-dihydroxyphenyl acetic acid  Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with DOPAC. (3, 4-dihydroxyphenyl acetic acid ) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to DOPAC. (3, 4-dihydroxyphenyl acetic acid ) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of DOPAC. (3, 4-dihydroxyphenyl acetic acid ) in the samples can be determined.