The AffiELISA® Human HE4 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Epididymal protein 4 (HE4) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Epididymal protein 4 (HE4) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Epididymal protein 4 (HE4) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Epididymal protein 4 (HE4) in the samples can be determined.
AffiELISA® Human HE4 Kit
Epididymal protein 4
Epididymis Protein 4 - dJ461P17.6 - EDDM4 - epididymal protein 4 - Epididymal secretory protein E4 - Epididymis specific whey acidic protein type four disulfide core - HE 4 - Major epididymis specific protein E4 - Major epididymis-specific protein E4 - MGC57529 - Putative protease inhibitor WAP5 - WAP 5 - WAP domain containing protein HE4 - WAP domain containing protein HE4 V4 - WAP four disulfide core domain 2 - WAP four disulfide core domain protein 2 - WAP four-disulfide core domain protein 2 - WAP5 - WFDC 2 - WFDC2 - WFDC2_HUMAN
Human
Sandwich ELISA
Serum, plasma and other biological fluids
Ovarian cancer .