AffiELISA® Mouse FYN Kit: High-Resolution FYN Oncogene Related To SRC/FGR/YES ELISA Detection

The AffiELISA® Mouse FYN Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain FYN Oncogene Related To SRC/FGR/YES (FYN) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of FYN Oncogene Related To SRC/FGR/YES (FYN) in the samples can be determined.