AffiELISA® Mouse GIP Kit: High Sensitivity Gastric Inhibitory Polypeptide ELISA Detection

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CAT# AFG-E3273
Size: 96 Tests

389.00 389.0 USD 389.00

389.00

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The AffiELISA® Mouse GIP Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Gastric Inhibitory Polypeptide (GIP) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Gastric Inhibitory Polypeptide (GIP) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Gastric Inhibitory Polypeptide (GIP) in the samples can be determined.

AffiELISA® Mouse GIP Kit

Gastric Inhibitory Polypeptide

Incretin hormone - Glucose Dependent Insulinotropic Peptide

Mouse

Competitive Inhibition ELISA

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Endocrinology, Gastroenterology, Hormone metabolism.