AffiELISA® Human HbA1c Kit: High-Resolution Glycated Hemoglobin A1c ELISA Detection

The AffiELISA® Human HbA1c Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Glycated Hemoglobin A1c (HbA1c) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Glycated Hemoglobin A1c (HbA1c) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Glycated Hemoglobin A1c (HbA1c) in the samples can be determined.