The AffiELISA® Mouse GAP43 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Growth Associated Protein 43 (GAP43) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Growth Associated Protein 43 (GAP43) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Growth Associated Protein 43 (GAP43) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Growth Associated Protein 43 (GAP43) in the samples can be determined.
AffiELISA® Mouse GAP43 Kit
Growth Associated Protein 43
F1 - B-50 - PP46 - Neuron Growth-Associated Protein 43 - Neuromodulin - Axonal Membrane Protein GAP-43 - Protein F1 - Calmodulin-Binding Protein P-57 - Neural Phosphoprotein B-50
Mouse
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Neuroscience, Developmental science.