The AffiELISA® Human ISG15 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Interferon Stimulated Gene 15 (ISG15) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Interferon Stimulated Gene 15 (ISG15) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Interferon Stimulated Gene 15 (ISG15) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Interferon Stimulated Gene 15 (ISG15) in the samples can be determined.
AffiELISA® Human ISG15 Kit
Interferon Stimulated Gene 15
G1P2 - IFI15 - ISG15 - Interferon Alpha-Inducible Protein clone IFI-15K - Interferon Induced Protein 15 - Interferon-induced 17 kDa protein - Ubiquitin Cross Reactive Protein - UCRP
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Cytokine.