AffiELISA® Chicken IL17 Kit: Max Sensitivity Interleukin 17 ELISA Detection

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CAT# AFG-E4525
Size: 96 Tests

454.00 454.0 USD 454.00

454.00

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The AffiELISA® Chicken IL17 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Interleukin 17 (IL17) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Interleukin 17 (IL17) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Interleukin 17 (IL17) in the samples can be determined.

AffiELISA® Chicken IL17 Kit

Interleukin 17

IL-17 - IL17A - CTLA8 - IL-17A - Cytotoxic T-Lymphocyte-Associated Protein 8

Chicken

Competitive Inhibition ELISA

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Cytokine, Infection immunity.