The AffiELISA® Mouse KEAP1 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1 (KEAP1) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Kelch Like ECH Associated Protein 1 (KEAP1) in the samples can be determined.
AffiELISA® Mouse KEAP1 Kit
Kelch Like ECH Associated Protein 1
INrf2 - KLHL19 - Cytosolic inhibitor of Nrf2 - Kelch-like protein 19
Mouse
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Signal transduction.