AffiELISA® Pan LTE4 Kit: High Sensitivity Leukotriene E4 ELISA Detection

The AffiELISA® Pan LTE4 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Leukotriene E4 (LTE4) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Leukotriene E4 (LTE4) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Leukotriene E4 (LTE4) in the samples can be determined.