The AffiELISA® Rat RIPK3 Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 3 (RIPK3) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Receptor Interacting Serine Threonine Kinase 3 (RIPK3) in the samples can be determined.
AffiELISA® Rat RIPK3 Kit
Receptor Interacting Serine Threonine Kinase 3
RIP3 - RIP-like protein kinase 3 - Receptor-interacting protein 3
Rat
Sandwich ELISA
Tissue homogenates, cell lysates and other biological fluids
Enzyme and Kinase.