AffiELISA® Rat RAGE Kit: Max Sensitivity Renal Tumor Antigen ELISA Detection

The AffiELISA® Rat RAGE Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Renal Tumor Antigen (RAGE) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Renal Tumor Antigen (RAGE) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Renal Tumor Antigen (RAGE) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Renal Tumor Antigen (RAGE) in the samples can be determined.