AffiELISA® Rat T4 Kit: High Sensitivity Thyroxine ELISA Detection

The AffiELISA® Rat T4 Kit uses competitive inhibition enzyme immunoassay technique. The provided microtiter plate comes pre-coated with Thyroxine (T4) protein. To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Thyroxine (T4) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. Afterward, the TMB substrate solution is added, initiating an enzyme-substrate reaction. To conclude the reaction, a sulphuric acid solution is introduced, causing a color change that can be measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Thyroxine (T4) in the samples can be determined.