The AffiELISA® Human P4HTM Kit uses Sandwich enzyme immunoassay. The provided microtiter plate comes pre-coated with an antibody specific to Transmembrane prolyl 4-hydroxylase (P4HTM) . To perform the assay, standards or samples are added to designated wells on the microtiter plate. Subsequently, a biotin-conjugated antibody specific to Transmembrane prolyl 4-hydroxylase (P4HTM) is introduced. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well, followed by an incubation period. After TMB substrate solution is added, only those wells that contain Transmembrane prolyl 4-hydroxylase (P4HTM) , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. By comparing the optical density (OD) of the samples to the standard curve, the concentration of Transmembrane prolyl 4-hydroxylase (P4HTM) in the samples can be determined.
AffiELISA® Human P4HTM Kit
Transmembrane prolyl 4-hydroxylase
EGLN4 - FLJ20262 - HIF prolyl hydroxylase PH4 - HIF-PH4 - HIF-prolyl hydroxylase 4 - HIFPH4 - HPH-4 - Hypoxia inducible factor prolyl 4 hydroxylase - Hypoxia inducible factor prolyl hydroxylase - Hypoxia-inducible factor prolyl hydroxylase 4 - P4H TM - P4H with transmembrane domain - P4H-TM - P4htm - P4HTM_HUMAN - PH 4 - PH4 - PHD4 - Proline 4 hydroxylase - Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) - Prolyl hydroxlase domain containing 4 - Transmembrane prolyl 4-hydroxylase -
Human
Sandwich ELISA
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Cardiovascular, Epigenetics and Nuclear Signaling, Cancer.